Performance evaluation of four 25-hydroxyvitamin D assays to measure 25-hydroxyvitamin D2
نویسندگان
چکیده
منابع مشابه
Assay for plasma 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 by "high-performance" liquid chromatography.
We describe a new, rapid, and simple column-chromatographic procedure for 25-OH vitamin D3 in plasma. The vitamin is extracted by use of Sep-pak C18 (octadecyl alkylated silicic acid), a short factory-packed reversed-phase column, and 25-OH vitamin D2 + 25-OH vitamin D3 fraction is eluted with methanol/water. The 25-OH D2 and 25-OH D3 are then well resolved on a high-resolution 3-micrometer sil...
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Although immunoassays in measuring 25-hydroxyvitamin D [25(OH)D] have been improved recently, relatively large differences are still seen between results of 25(OH)D measured by immunoassays and by liquid chromatography-tandem mass spectrometry (LC-MS/MS). In the present studies, we compared two immunoassays with LC-MS/MS for measuring 25(OH)D concentrations. Concentrations of 25-hydroxyvitamin ...
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The recently reported difficulties experienced by Quest Laboratories (1 ) have once again highlighted concerns about the reliability of 25-hydroxyvitamin D (25-OHD) results, particularly those generated by liquid chromatography–tandem mass spectrometry (LC-MS/ MS), the method used by Quest. In the New York Times article of January 8, 2009 (1 ), a spokesman for Quest admitted that some erroneous...
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BACKGROUND There is increasing evidence that vitamin D deficiency is common and has been associated with several non-bone related outcomes, including insulin resistance, type 2 diabetes and cardiovascular disease. The influences of gender, puberty, and adiposity on serum hydroxyvitamin D (25-OH-D) levels and the relationship between 25-OH-D and insulin resistance in obese children were studied....
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Low vitamin D status is common globally and is associated with multiple disease outcomes. Understanding the correlates of vitamin D status will help guide clinical practice, research, and interpretation of studies. Correlates of circulating 25-hydroxyvitamin D (25(OH)D) concentrations measured in a single laboratory were examined in 4,723 cancer-free men and women from 10 cohorts participating ...
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ژورنال
عنوان ژورنال: Clinical Biochemistry
سال: 2015
ISSN: 0009-9120
DOI: 10.1016/j.clinbiochem.2015.05.021